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| 克痛方对急性闭合性软组织损伤的治疗作用与机制 |
| The Therapeutic Effect and Mechanism of Ketong Recipe on Acute Closed Soft Tissue Injury |
| 投稿时间：2025-02-25 修订日期：2025-02-25 |
| 中文关键词: 克痛方 急性闭合性软组织损伤 炎症因子 白细胞介素 |
| 英文关键词: Ketong Recipe acute closed soft tissue injury Inflammatory factors Interleukins |
| 基金项目:2023年全国中药特色技术传承人才培训项目(项目编号:T20234832005)、浙江省中医药科技计划项目(编号:2023ZF174,2024ZL1122,2023ZL728) |
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| 中文摘要: |
| 目的:研究克痛方治疗急性闭合性软组织损伤的治疗作用。方法:采用重物高空垂直下落造成打击伤建立大鼠急性软组织损伤模型。144只SD大鼠,随机分为正常组、模型组、克痛方组、扶他林组,每组36只,除空白组外将其余各组大鼠固定在软组织打击器上通过打击锤敲打造成大鼠急性闭合性软组织损伤,在造模当天每组大鼠外涂给予相应的药物。给药第1、3、7天时,每组每次脱颈处死12只大鼠,检测血液粘度以及血浆粘度,测定血浆纤维蛋白原含量、血小板聚集率、红细胞压积、红细胞聚集指数(AI)；HE染色观察肌肉组织病理病变情况；采用酶联免疫法( ELISA) 检测大鼠损伤组织以及血清中白细胞介素1β(IL-1β)、白细胞介素8(IL-8)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)的含量；Q-PCR反应检测白IL-1β、IL-8、IL-6、TNF-α的mRNA的含量；蛋白印迹法( Western bloting) 测定大鼠损伤组织中P65、p-P65、P50、p-P50、IKBα蛋白表达。结果:与模型组比较,从第三天开始,克痛方能够有效的降低大鼠血清中IL-1β、IL-8、IL-6、TNF-α相关炎症因子的水平；提高血液粘度以及血浆粘度,增加血浆纤维蛋白原含量、血小板聚集率、红细胞压积、红细胞聚集指数等,能够降低肌肉病变总评分,改善组肌肉组织出血等病变；q-PCR与 Elisa蛋白结果显示,与模型组相比,克痛方处理组能够有效的降低大鼠血清中IL-1β、IL-8、IL-6、TNF-α相关炎症因子的水平mRNA水平以及相应的蛋白水平。结论:克痛方通过降低血清以及受损组织中的IL-1β、IL-8、IL-6、TNF-α水平及抑制肌肉组织P65、p-P65、P50、p-P50、IKBα等因子相关基因、蛋白水平的表达,抑制组肌肉组织出血、改善血流变学指标发挥治疗急性闭合性软组织损伤的作用。 |
| 英文摘要: |
| Objective: To study the herapeutic effects of Ketong Recipe in rats with acute closed soft tissue injury. Methods: 144 rats were randomly divided into 4 groups: blank control group, model control group, Ketong Recipe group and voltalin ointment group, Except for the blank group, the rats in other groups were fixed on the soft tissue percussion device and made into acute closed soft tissue injury by percussion hammer. after the modeling, the rats in each group applied corresponding drugs in vitro, once a day, continuously administered for 7 days. After the administration on the 1st, 3rd and 7th day,12 rats were dissected in each group, and the blood was taken from the abdominal aorta. The whole blood viscosity, plasma viscosity and hematocrit , the |
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