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| 化湿宣肺颗粒治疗溃疡性结肠炎:网络药理学和生物信息学揭秘分子机制 |
| Huashi Xuanfei Granules in the treatment of ulcerative colitis: network pharmacology and bioinformatics revealing |
| 投稿时间：2024-12-20 修订日期：2024-12-20 |
| DOI： |
| 中文关键词: 化湿宣肺颗粒 溃疡性结肠炎 网络药理学 生物信息学 炎症 |
| 英文关键词: Huashi Xuanfei Granules Ulcerative colitis Network Pharmacology Bioinformatics Inflammationmolecular mechanisms |
| 基金项目:化湿宣肺颗粒治疗普通型新冠肺炎临床前研究 编号(2021C03033)。 |
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| 中文摘要: |
| 摘要 目的 通过网络药理学和生物信息学方法，以及体外验证，探讨化湿宣肺颗粒（HSXF）治疗溃疡性结肠炎（UC）的关键靶点及潜在药理机制。方法 从公共数据库获取HSXF和UC相关数据，使用Cytoscape软件筛选核心基因，并利用R语言进行GO富集、KEGG通路、免疫浸润分析及模型诊断，探讨HSXF治疗UC的潜力与机制。结果 共筛选出669个HSXF相关基因和800个UC差异基因。GO和KEGG分析显示主要富集于炎症和免疫相关生物过程与通路。免疫浸润分析表明，HSXF可靶向调节T细胞和巨噬细胞以治疗UC。模型诊断结果显示核心基因具有较高的诊断价值。qPCR结果证实HSXF能够抑制IL1B、MMP1、MMP9和PTGS2的表达。结论 HSXF可通过调控相关信号通路及免疫细胞，靶向IL1B、MMP9、MMP2和PTGS2，发挥对UC的治疗作用。 |
| 英文摘要: |
| Objective: To investigate the key targets and potential pharmacological mechanisms of Huashi Xuanfei Granules (HSXF) in treating ulcerative colitis (UC) through network pharmacology and bioinformatics methods and in vitro validation. Methods The data related to HSXF and UC were obtained from public databases, and the core genes were screened by Cytoscape software, and GO enrichment, KEGG pathway, immune infiltration analysis, and model diagnosis were performed by R language, to explore the potential and mechanism of HSXF in the treatment of UC. Results A total of 669 HSXF-related genes and 800 UC differential genes were screened. GO and KEGG analysis showed it was mainly enriched in inflammation and immune-related biological processes and pathways. Immune infiltration analysis showed that HSXF could target and regulate T cells and macrophages to treat UC. The diagnostic results of the model showed that the core gene had a high diagnostic value. The qPCR results confirmed that HSXF could inhibit the expression of IL1B, MMP1, MMP9, and PTGS2. Conclusion HSXF can play a therapeutic role in the treatment of UC by regulating related signaling pathways and immune cells to target IL1B, MMP9, MMP2, and PTGS2. |
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